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Figure 5 | BMC Cell Biology

Figure 5

From: An initial biochemical and cell biological characterization of the mammalian homologue of a central plant developmental switch, COP1

Figure 5

Subcellular localization of MmCOP1 deletion mutant proteins (A) Diagrams of the deletion constructs of MmCOP1 used for the localization studies. ΔN70, ΔRING, N346, N280 and N200 are shown in comparison to the full-length MmCOP1 protein (WT), with the locations of the RING-finger (RING), Coiled coil (Coil) and WD40 repeats (WD40) highlighted. The amino acid coordinates at the start and end of each deletion protein version are marked. These sets of COP1 mutants are fused to the C-terminals of GFP or FLAG epitope. The subcellular localization pattern of each protein is indicated on the right. C, cytoplasmic; N, nuclear; C/N, both cytoplasmic and nuclear. (B) GFP fluorescence study of the subcellular localizations of GFP-ΔN70, GFP-ΔRING, GFP-N346, GFP-N280 and GFP-N200. ΔN70 (I-III) are representative of three different localization patterns of cells expressing GFP-ΔN70. The studies were carried out in COS7 cells. The same magnification was used for all images and the scale bar for 10 micrometer was indicated in the image for ΔN70 (III).

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