Figure 4
Effect of gadolinium on ERK1/2 responses. A: Hepatocytes cultured for 3 h were pretreated with gadolinium (100 μM) or EGTA (5 mM) for 15 min in the presence of timolol (10 μM) prior to stimulation with norepinephrine (10 μM) for 5 min before cells were harvested. Results are activity measurements of ERK1/2 given as pmol 32P incorporated into MBP/mg protein representing mean ± S.E.M. of three experiments. B: Immunoblot showing the effect of pretreatment with gadolinium (100 μM) for 15 min on A23187 (10 μM) induced ERK1/2 response. Antibody against dually phosphorylated ERK1/2 was used.