Figure 6

GHFT1-5 cells were transfected with the A, Sham, GFP-C/EBP and ΔLZ expression vectors or B, Sham, C/EBP-GFP and DBD expression vectors and treated with nocodazole. The proportions of cells in G1, S and M phase were determined and plotted as the mean +/- standard deviation from six independent experiments for both A and B. No C/EBP, the subpopulation of cells with background levels of green fluorescence (i.e. did not express GFP-linked C/EBPα). Data from No C/EBP cells are shown adjacent to the green fluorescent cells for each expression construct. Statistically significant differences in the proportion of green fluorescent cells in G1, S or G2/M, relative to the proportions determined for the sham-transfected cells, are indicated (One-way ANOVA: ^**, p < 0.01; ^*p < 0.05; -, no difference).