Figure 7

TGF-β1 suppresses Hyal-1 and Hyal-2-increased TNF cytotoxicity in L929 cells. L929 cells stably expressing GFP, GFP-Hyal-1 or GFP-Hyal-2 were cultured in 96-well plates overnight, treated with TGF-β1 (2 ng/ml) for 2 hr, and then cotreated with TNF-α (5–40 ng/ml) for 24 hr. TGF-β1 inhibited TNF-α killing of L929 cells expressing GFP, and GFP-Hyal-1 or GFP-Hyal-2 (mean ± β1 inhibited TNF-α killing of the control GFP L929 cells to a greater extent (50–70%) than the GFP-Hyal-1 or GFP-Hyal-2 cells (30–50%).