Lipopolysaccharide enhances the cytotoxicity of 2-chloroethyl ethyl sulfide

Table 1 IL-1β Enhances the Cytotoxicity of CEES to RAW264.7 Macrophages (24 hours)¹

Assay²	Control	LPS	IL-1β	CEES	LPS+CEES	IL1β +CEES
PI(%) ²	0.0 ± 0.0^a	10.6 ± 2.1^a	10.2 ± 4.1^a	64.8 ± 16.9^b	120.8 ± 2.3^c	111.1 ± 3.9^c
MTT³	1.61 ± 0.1^b	1.20 ± 0.04^a	1.45 ± 0.03^b	1.13 ± 0.16^a	0.25 ± 0.07^c	0.74 ± 0.07^d

¹LPS was used at 25 ng/ml, IL-1β at 50 ng/ml and CEES 500 μM. Values are mean ± SD and means (in a given row) not sharing the same superscript letter are significantly different (p < 0.05). ²PI(%) is the percent of propidium iodide fluorescence over that of the control cells. ³The MTT levels are given as 575 nm absorbance units.

ISSN: 2661-8850