Lipopolysaccharide enhances the cytotoxicity of 2-chloroethyl ethyl sulfide

Table 2 TNF-α Enhances the Cytotoxicity of CEES to RAW264.7 Macrophages (24 hours)¹

Assay²	Control	LPS	TNF-α	CEES	LPS+CEES	TNF-α +CEES
PI (%)	0.0 ± 0.0^a	0.8 ± 0.5^a	3.3 ± 1.3^a	15.6 ± 4.1^b	62.6 ± 1.4^c	34.1 ± 7.6^b
MTT³	1.68 ± 0.08^a	1.61 ± 0.04^a	1.55 ± 0.08^a	1.37 ± 0.09^a	0.32 ± 0.05^b	1.08 ± 0.18^c

¹LPS was used at 25 ng/ml, TNF-α at 50 ng/ml and CEES 500 μM. Values are means ± SD and means (in a given row) not sharing the same superscript letter are significantly different (p < 0.05). ²PI(%) is the percent of propidium iodide fluorescence over that of the control cells. ³The MTT levels are given as 575 nm absorbance units.

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