Figure 6
Association of the MyHC-like molecules with intermediate filament-containing structures. N3.36-antigen (A,B,F red; D green), A4.74-antigen (A-C, green; G, red), A4.840-antigen (C, red), phalloidin (E), pan-keratin (F, green) and keratin 5 (G, green) in cryosections of rat epidermis. Longitudinal section of hair follicle from partially-trypsinised P7 rat skin showing the single layer of N3.36-labelled cells of the hair bulb (A, red), immediately external to the A4.74-labelled layer (A, green). At higher magnification, the same distribution is observed in confocal cryosection of an intact rat hair follicle with A4.74-antigen present in the layer internal to the N3.36-antigen (B). A4.74-labelled fibrils (C, green) appear to course through the cytoplasm of cells of the root sheath linking to regions of A4.840-labelled plasma membranes (C, red). Dual localisation of N3.36-reactive fibrils by immunofluorescence (D) and actin microfibrils with rhodaminated-phalloidin (E) show that the major location of the MyHC-like molecule is not on actin cytoskeleton. Similar results were obtained with A4.74 and phalloidin. The N3.36-antigen (red, F) is associated with the outermost layer of keratin-containing cells (green, F). These cells also contain keratin 5 (green, G), based on their position immediately external to the A4.74-reactive cell layer (red, G).