BMC Molecular and Cell Biology

Table 1 Summary of antibodies, their known MyHC epitopes and epithelial reactivity.

From: Intermediate filament-co-localized molecules with myosin heavy chain epitopes define distinct cellular domains in hair follicles and epidermis

Antibody	Muscle reactivity			Epithelial reactivity
	MyHC Gene	Epitope location	Expression timing	Surface epidermis	Hair¶follicle	Gut	Non-neural retina	Bladder
A4.840	Slow/β	Rod	Embryo onwards	All	IRS/ ORS	All	+	+
	HRMC§	H	HRMC	HRMC	HRMC*	R	R	R
N3.36	Neo/adult fast	N-terminal 25 aa	Fetal/ postnatal	Some basal cells	ORS	Crypt base	+	+
	HRM	H	HRM	HR (not M)	HR (not M)	R	R	R
N2.261	Slow/β + Fast IIA	N-terminal 25 aa	Neonatal onwards	Basal	-	-	+	+
	HRM	H(β)	RM	HR	HR	R	R	R
A4.74	Fast IIA	Unknown	Postnatal onwards	-	IRS	-	-	-
	HRM		RM	HRM	HRM	R	R	R
N1.551	Neonatal + Adult Fast	Unknown	Fetal onwards	-	Bulge? neck cell cluster	-
	HRM		HRM	R	R	R
A4.951	Adult slow/β	N-terminal 25 aa	Postnatal onwards	Weak in fetus only	Bulge? neck cell cluster	-
	HRM	H	RM	R	R	R
A4.1025	All known	20 kd / 25 kd junction/ ATP site	Embryo onwards	-	-	-	-	-
	HRMCDZX	H	HRMCDZX	HRM	HRM	R	R	R

Note that three antibodies (N3.36, N2.261 and A4.951) that detect MyHC-like antigens in epithelia have MyHC epitopes in the N terminus of the S1 fragment of the head. X-ray crystallography does not reveal a coiled coil (or any other conserved structure) in this region. §Letters under each entry indicate the species in which reactivity is observed: H human, R rat, M mouse, C chick, D Drosophila, Z zebrafish, X Xenopus. Absence of a species indicates data not available unless specifically noted otherwise. ¶IRS inner root sheath, ORS Outer root sheath, * in feather germ.

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ISSN: 2661-8850

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