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Figure 1 | BMC Cell Biology

Figure 1

From: Overexpression of the human MNB/DYRK1A gene induces formation of multinucleate cells through overduplication of the centrosome

Figure 1

Subcellular localization of GFP-MNB/DYRK1A (A) and FLAG-tagged MNB/DYRK1A (B) in HeLa cells. HeLa cells were transfected with constructs encoding GFP, GFP-NLS, GFP-MNB/DYRK1A, or FLAG epitope-tagged MNB/DYRK1A as described in "Materials and Methods." The cells were then fixed, stained with 1 μM TOTO-3 for the counterstaining of DNA, and observed by confocal laser scanning microscopy. GFP or FITC and DNA are displayed in green and blue, respectively. Merged images are also shown in the third photo from the left. Phase-contrast images are shown at the right. The data are representative of those of 5 independent experiments. Scale bar, 10 μm.

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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