Figure 3From: Overexpression of the human MNB/DYRK1A gene induces formation of multinucleate cells through overduplication of the centrosomeHigh-level expression of GFP-MNB/DYRK1A in multinucleate cells. HeLa cells were transfected with cDNA encoding GFP-MNB/DYRK1A. At 48 hours after transfection, the cells were fixed, stained with anti-MNB antibody and TOTO-3, and observed with a confocal laser scanning microscope as described in "Materials and Methods." Scale bar, 10 μm.Back to article page