IGF-1/IGFBP-1 increases blastocyst formation and total blastocyst cell number in mouse embryo culture and facilitates the establishment of a stem-cell line

Table 1 The effect of IGF-1 upon the developmental competence of in vivo fertilized mouse zygotes.

Culture medium	Total n	Zygotes arrested in one-cell to morula stage (%)	Zygotes developed to Blastocyst (%)
			Blastocyst (%)	Hatched blastocyst (%)
kSOM/AA with IGF-1	106	12(11.3)	94(88.7) *
			43(40.6) **	51(48.1) **
kSOM/AA without IGF-1	83	15(18.1)	68(81.9) *
			31(37.3) **	37(44.6) **

IGF-1: 100 ng/ml. The results were pooled from 5 repeats of experiments. * P = 0.043 ** P > 0.05

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