Overexpression of TLK1B-KD reduces the level of phosphorylated histone H3. a. Expression of wt and KD mutant TLK1B assessed by labeling the proteins with [35S]methionine and immunoprecipitation . b. Western blot of histone H3 and phosphorylated H3 in the indicated cell lines, as explained in Methods. The bar-graph is the plot of three different experiments analyzed by densitometry, and shown as the average for each cell line and standard deviation.