Figure 5From: A dominant negative mutant of TLK1 causes chromosome missegregation and aneuploidy in normal breast epithelial cellsTransfection of siRNA against TLK1 mRNA results in slow progression from G1. Cells were transfected with duplex siRNA oligos, control (scrambled sequence: panel A and B) or TLK1 (panel C and D). Forty-eight hours after transfection, cells were treated (panel B and D), or not, with 1 μM nocodazole for 18 h. The inserted panel E is a western blot of TLK1 with and without treatment with siRNA.Back to article page