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Figure 5 | BMC Cell Biology

Figure 5

From: FGF signaling inhibits the proliferation of human myeloma cells and reduces c-myc expression

Figure 5

FGF Signaling Induces Cell Death in OPM2 Cells. A) RPMI 8226 myeloma cells were cultured in serum free medium for 24 hours or serum containing medium plus 10 uM dexamethasone (last three columns) in the presence of the indicated factors. The number of dead cells was evaluated using trypan blue exclusion two days after dexamethasone treatment. B) FGF signaling does not protect ARP-1 myeloma cells from dexamethasone-induced apoptosis. Conditions as in panel A. Pre-treatment consisted of pre-incubation of the cells with IL-6 for one hour, followed by death inducer. C) OPM2 cells were cultured in serum free medium for one day in the presence or absence of FGF1 and cell cycle analysis of ethidium bromide stained nuclei was performed. Results are representative of four independent determinations; p < .01 Student's paired t test. Red trace shows results with 100 ng/ml FGF1 as compared to heparin only (blue trace). Inset shows quantification of sub G0 DNA content for 10 and 100 ng/ml FGF1 as compared to heparin alone and a magnified version of the sub G0 region shown in the FACS.

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