Figure 3

ADP-ribosylation of Rho alters HPB-ALL spreading on β1 integrin substrates. Cells were plated on BSA (panels 1–3), Fn (panels 4–6), VCAM-1 (panels 7–9), anti-β1 mAb 33B6 (panels 10–12), or poly-L-lysine (panels 13–15). HPB-ALL cells were electroporated with 25 μg/ml C3 (right column), 25 μg/ml BSA (middle column), or untreated (left column). Initial magnification was 200 ×. Experiments in panels 1–9 and panels 10–15 were performed separately. The images are representative of at least four separate experiments.