Wg signaling activity is impaired with the loss of either Arr or DFz2 function by dsRNA interference. S2 cells were treated with the following dsRNAs: Aco as a control (A-C), Arr (A-C), DFz2 (A, C), and Dsh (B). The next day, cells were transfected with the following expression plasmids: Wg (A), Wg and DFz2 (B) and β-cateninS37A (C) together with the reporters. The level of fold activation was set to 100% for cells treated with control dsRNAi, and the actual value is indicated above the figures. The responses of the transfected S2 cells treated with different dsRNAs are based on the percentage of fold activation compared with the control. All experiments were done in triplicate; error bars represent standard deviations.