LRPΔN activate the pathway independent of DFz2 and Dsh and N-terminus of LRP is required for the synergistic activity of Wg and DFz2. S2 cells were treated with the following dsRNAs: control Aco (A-C), DFz2 (A, B), Dsh (A, B), Arm (A, B) and Arr (C). The next day cells were transfected with indicated expression plasmids. Fold activation values were measured relative to the levels of luciferase activity in cells transfected with empty vectors and normalized by Renilla luciferase activities. The level of fold activation was set to 100% for cells treated with Aco dsRNA and the actual values are indicated above the figures (A, B). All experiments were done in triplicate; error bars represent standard deviations.