Figure 2

Arp3 is de-localized with BDM treatment. (A) BS-C-1 cells stained for Arp3 and actin after 1 minute of treatment with a media control, BDM, cytochalasin D, or both BDM and cytochalasin D. BDM treatment leads to de-localization of Arp3 and loss of the brightly stained leading edge actin network. Arp3 is not de-localized with cytochalasin D treatment or a combination of cyto D and BDM, suggesting that actin dynamics may be required for de-localization. The percentage of cells with the phenotype pictured is noted for each treatment (n =100 cells). Bar, 50 um. (B) Higher magnification of control and two BDM treated fields show actin and Arp3 staining. Actin at the leading edge of control cells (arrow) is denser, suggestive of a well-organized network, and has fewer bundled projections as compared to BDM treatment. Bar, 20 um.