Figure 2
From: Identification of a distinct class of cytoskeleton-associated mRNAs using microarray technology
Actin microfilament integrity is required for the cytoskeleton-association of transcripts. HL-60 cells were treated with latrunculin B (0.1 μg/ml) to depolymerize actin microfilaments. RT-PCR analysis reveals that cytoskeleton-associated transcripts shift to the cytosolic fraction upon latrunculin B treatment, indicating that actin filaments were critical in maintaining the cytoskeleton-localization of these transcripts. (A similar distribution and requirement for actin cytoskeleton integrity was observed in RT-PPCR studies of protein 4.1 and thymosin-β4)