TY - JOUR AU - Aldonyte, Ruta AU - Jansson, Lennart AU - Janciauskiene, Sabina PY - 2004 DA - 2004/03/29 TI - Concentration-dependent effects of native and polymerised α1-antitrypsin on primary human monocytes, in vitro JO - BMC Cell Biology SP - 11 VL - 5 IS - 1 AB - α1-antitrypsin (AAT) is one of the major serine proteinase inhibitors controlling proteinases in many biological pathways. There is increasing evidence that AAT is able to exert other than antiproteolytic effects. To further examine this question we compared how various doses of the native (inhibitory) and the polymerised (non-inhibitory) molecular form of AAT affect pro-inflammatory responses in human monocytes, in vitro. Human monocytes isolated from different donors were exposed to the native or polymerised form of AAT at concentrations of 0.01, 0.02, 0.05, 0.1, 0.5 and 1 mg/ml for 18 h, and analysed to determine the release of cytokines and to detect the activity of NF-κB. SN - 1471-2121 UR - https://doi.org/10.1186/1471-2121-5-11 DO - 10.1186/1471-2121-5-11 ID - Aldonyte2004 ER -