Figure 4

A and B. Monitoring of NFκB p50 activation in monocytes after 18 h of exposure to different concentrations of native or polymerized AAT by ELISA-based TransAM kit^AM. Cell nuclear extracts were prepared from monocytes alone or treated with different concentrations of AAT. NFκB activity assay was performed in 96-well plates with 10 μg of cell extract per well. Absorbance was measured by spectrophotometry at 450 nm. Specificity controls were performed by adding a molar excess (20 pmol/well) of mutant NFκB oligonucleotide (the positive signal remained unaffected) and wild-type NFκB oligonucleotide (a signal was abolished). A and B represent results from two independent experiments. Each point represents the mean of two experiments.