Immunostaining for lamin A/C is deficient in lymphoblastoid cells with mutation LMNA R377H. Indirect immunofluorescence microscopy of lymphoblastoid cells from patient cells (a-c) and control cells (d-f) after staining with antibodies against emerin (a,d), lamin A/C (b,e) and lamin B (c,f). Lamin A/C staining is less intense in patient cells (b) compared with the control (e). The corresponding Hoechst fluorescence in shown (a'-c' and d'-f'). Bar: 10 µm.