Immunostaining for lamin A/C is deficient in frozen muscle sections of the AD-EDMD patient 99-3. Immunolabelling of frozen muscle sections from patient 99-3 (a-c) and control (d-f) was performed for emerin (a,d), lamin A/C (b,e) and lamin B (c,f). Lamin A/C staining is less intense in patient (arrow in b) compared with the control (e). The corresponding phase-contrast images (a"-c"; d"-f") and Hoechst fluorescences (a'-c'; d'-f') are shown. Bars (c",f") 10 µm.