Figure 2

Flow cytometric analysis of cells treated with SSW smoke. Cells were treated with 1:9 SSW smoke or with staurosporine and analyzed by flow cytometry to determine the forward- and side-scattering properties of the cells. (A&B) Untreated and SSW-treated cells show similar pattern of forward- and side-scattering properties. (C) Staurosporine treated cells (positive control) showed more cells with lower forward-scattering properties than either control or SSW-treated cells suggesting that SSW smoke is not causing cell death and that the overall structure of the cell is normal. The graphs represent 10,000 events. (D) Acridine-orange and ethidium bromide staining; cells showed normal morphology, and no blebbing of the nucleus or plasma membrane was observed. SSC = side-scatter, FSC = forward-scatter. Figures are representative of at least 3 repeated studies. Scale bar = 20 μm.