Figure 3

Effects of SSW smoke on fibroblast growth. (A) Cells were treated for 18 hours with SSW smoke solution and the total cell number was counted using a Coulter counter with a specified particle size of 7 μm to 20 μm. There was no significant difference between controls and treated cells. (B) Primary fibroblasts were plated in 96-well plates and allowed to grow to confluency and BrdU alone or BrdU plus SSW were added to the cultures and the cells were allowed to incorporate the BrdU for the indicated time points. At both 6 and 18 hours, SSW-treated cells showed a significant decrease in BrdU incorporation when compared to the control. Experiments are performed at least two times with different batches of primary fibroblasts. OD = Optical Density.