Figure 6

Microscopic analysis of cells treated with SSW smoke. (A) SSW-treated cells developed numerous vacuoles in the cytosol. Inset shows a higher magnification of the vacuoles. (B-F) Cells treated with SSW for 4 hours were fixed and prepared for Transmission Electron Microscopy (TEM). We observed that the cells are still morphologically intact (B) except for the endomembrane system, which is beginning to show swelling (arrows). However, most organelles, such as the mitochondria (C) and the nucleus (D) look normal, whereas the endomembrane system is irregularly shaped and shows signs of swelling (E). (F) Endomembrane system of untreated cells. Scale bars = 20 μm in (A), 5 μm in (B) and 0.6 μm in (C-F).