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Figure 2 | BMC Cell Biology

Figure 2

From: Down-regulation of the M6P/IGF-II receptor increases cell proliferation and reduces apoptosis in neonatal rat cardiac myocytes

Figure 2

Effect of the ribozyme on M6P/IGF2R mRNA in cardiac myocytes. (A) A representative RT-PCR result showing the levels of M6P/IGF2R mRNA (lanes 1 and 2) and the corresponding amounts of β-actin mRNA (lanes 3 and 4) in the ribozyme-treated myocytes (lane 1, lane 3) and in the control cells (lane 2, lane 4). (B) Bar chart showing percent change in M6P/IGF2R mRNA of the ribozyme-treated cells relative to the mRNA fraction in the control cells (normalized to β-actin). The data are average ± SE of at least three independent experiments (*, P < 0.05 versus control). (C) RPA analysis of M6P/IGF2R down-regulation by ribozyme treatment (lane 1, ribozyme-treated; lane 2, control) (see methods for procedure). A probe targeting the GAPDH gene was used as an internal control. (D) Bar chart showing percent change in M6P/IGF2R mRNA of the ribozyme-treated cells relative to the mRNA fraction in the control cells (normalized to GAPDH). The data are average ± SE of at least four independent experiments (*, p < 0.05 versus control).

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