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Figure 2 | BMC Cell Biology

Figure 2

From: Assembly of the giant protein projectin during myofibrillogenesis in Drosophila indirect flight muscles

Figure 2

Projectin and other Z-band associated proteins show early colocalization during pupal myofibrillogenesis. Immunofluorescence microscopy images representing the staining pattern obtained at 36–40 hours ASP for projectin, several Z-band proteins, as well as F-actin. Pupae were aged and after dissection, the tissues were spread and processed for immunofluorescence as described in Methods. Corresponding left and right panels represent images from double immunostaining on the same cell. Panels A, C and E: projectin, panel B: Z(210), panel D: F-actin, panel F: kettin; panel G: α-actinin and panel H: Z(210). The five proteins follow a similar pattern of aggregates where I-Z-I proteins colocalize. Scale bar in A is for panels A through H and represents 10 μm.

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