Figure 1

Mapping the Ypt6 binding site on Sgm1 A. Coiled-coil probabilities of the Sgm1 protein sequence, calculated by the method of Lupas using the coils program. The regions analysed for binding are shown diagrammatically below. B. Immunoblot with anti-Protein A of the indicated portions of Sgm1 bound to and eluted from GST-Ypt6 in the GTP and GDP state. Input samples are yeast extract corresponding to about 1% of the total input. The intact protein A fusions are indicated by the arrowheads. Asterisks mark C terminal proteolytic fragments, still fused to protein A, that are common to both the full-length and 488–707 constructs; note that the smallest visible fragment corresponds closely to the 597–707 construct. Proteins apparently larger than the input in the 488–707 lanes presumably represent dimers and other aggregates.