Figure 1

Panel A. Schematic diagram demonstrating various gelsolin constructs generated in HIV-TAT-expression vector. Actin binding domain (S1-S6) and phosphoinositide binding domains (PBDs) are indicated in full-length gelsolin (FL-Gsn). Cloning was performed as described in the Methods Section. Phosphoinositides binding domains 1, 2, and 3 are shown in green color. Two-tandem repeats of first and second phosphoinositides binding domains (aa 133–169) were also cloned. This is represented as PBD (2) in the text. Panel B. Western analysis of the purified peptides using a HA antibody. Purified TAT-fused gelsolin peptides were subjected to 8% (lanes 1–3) and 15% SDS-PAGE (lanes 4–7). The blots were subjected to Western analysis with an antibody to HA. The results shown are representative of three independent experiments.