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Table 1 The effects of HA-TAT-protein transductions and OPN treatment on gelsolin/actin ratio and F-actin content of osteoclasts. F-actin content was measured by rhodamine-phalloidin binding in osteoclasts treated as indicated in the table. About 4–5 wells were used for each treatment. The experiment was repeated three times. The data presented are mean ± SE for three experiments.

From: Polyphosphoinositides-dependent regulation of the osteoclast actin cytoskeleton and bone resorption

 

Gelsolin/actin ratio

F-actin content

OCs transduced with

PBS

OPN

PBS

OPN

Untransduced

1.45 ± 0.15

0.82 ± 0.18••

1.0

3.5 ± 0.09***, *

Tat/FL-Gsn

1.3 ± 0.15

1.23 ± 0.08

2.5 ± 0.32 *

3.2 ± 0.81*

Tat/NH-Gsn

1.32 ± 0.13

1.35 ± 0.15

1.95 ± 0.05*

2.6 ± 0.06*

Tat/Mid-Gsn

1.38± 0.18

0.71 ± 0.06••

1.20 ± 0.11

3.1 ± 0.33 **, °°°

Tat/PBD

1.42 ± 0.16

1.37 ± 0.14

2.17 ± 0.22*

2.2 ± 0.2 *

Tat/PBD(2)

1.36 ± 0.11

1.44 ± 0.21

2.19 ± 0.10*

2.6 ± 0.23*

Tat/COOH-Gsn

1.35 ± 0.12

1.41 ± 0.14

2.11 ± 0.11*

2.8 ± 0.09*

HA-TAT

1.33± 0.09

0.73 ± 0.07••

1.092 ± 0.02

3.6 ± 0.15 °°°

  1. ••p < 001-OPN, Mid-Gsn/OPN, and HA-TAT/OPN-treated vs. respective PBS-treated osteoclasts °°°p < 0.0001 Mid-Gsn/OPN and HA-TAT/OPN-treated vs. HA-TAT/PBS-treated osteoclasts *p < 0.01-PBS, OPN-treated TAT-fusion proteins transduced osteoclasts vs. PBS-treated untransduced (-) and HA-TAT transduced control osteoclasts **p < 001-OPN-treated Mid-GSN transduced osteoclasts vs. PBS-treated Mid-Gsn transduced osteoclasts *** p < 0.0001-OPN-treated osteoclasts vs. untransduced PBS-treated (-) osteoclasts
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BMC Molecular and Cell Biology

ISSN: 2661-8850

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