Figure 1

Cytochalasin B stimulation of TNF-α primed neutrophils. Neutrophils were pre-incubated with TNF-α (25 ng/ml, 20 min, 37°C) after which they were stimulated with cytochalasin B The extracellular release of superoxide anions was measured by isoluminol-amplifled chemiluminescence (CL) given as Mcpm (10⁶ counts per minutes). The figure shows the kinetics of a representative experiment. The indicated value shows the mean peak value ± SD, n = 3. The ability of cytochalasin B to induce NADPH-oxidase activity was dependent on the priming concentration of TNF-α (inset; comparing the peak values of the responses at varying concentrations to the value obtained with 25 ng/ml of TNF-α).