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Figure 5 | BMC Cell Biology

Figure 5

From: Cytochalasin B triggers a novel pertussis toxin sensitive pathway in TNF-alpha primed neutrophils

Figure 5

Cyclosporin H sensitivity of cytochalasin B induced superoxide anion production in fMLF desensitized, WKYMVM desensitized or TNF-α primed neutrophils. Neutrophils were preincubated with fMLF (10-7M; 10 min A), WKYMVM (10-7M; 10 min B) or TNF-α (25 ng/ml; 20 min C). The cells were challenged with cytochalasin B in samples without (solid lines) and with cyclosporine H (final concentration in A, 10-7M and in B and C, 10-6M; dashed lines) that was added 1 min before cytochalasin B. The production of superoxide anions was measured by isoluminol-amplified CL as described above. The figure shows the kinetics of representative experiments and the ratios between the peak response in the absence and presence of cyclosporine H are given.

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