Figure 6
From: Cytochalasin B triggers a novel pertussis toxin sensitive pathway in TNF-alpha primed neutrophils
Dilution effects on cytochalasin B induced activation Neutrophils were incubated in the presence of fMLF (10-7M; A) or TNF-α (25 ng/ml; B) for 20 min at 37°C and then diluted in measuring vials containing the same concentration (high; solid lines) or no agonist (low; dashed lines), respectively. The cells were then immediately challenged with cytochalasin B. The production of superoxide anion was measured by isoluminol-amplified CL as described above. The figure shows the kinetics of representative experiments and the ratios, determined from the peak response in the presence of high and low concentrations of agonist.