Figure 7

Addition of non-primed cells to TNF-α primed cells and activation by cytochalasin B. Neutrophils incubated for 20 min at 37°C for 20 min were added to a population of TNF-α primed cells. The amount of superoxide release by the newly added cells was determined (A; dashed line). For comparision the NADPH-oxidase activity induced by cytochalasin B when added to the TNF-primed cells (A; solid line) is also shown. The NADPH-oxidase activity induced by cytochalasin B in a cell population where all the cells were primed with TNF-α for 20 min (B; solid line) was compared to that of a cell population where 50% of the cells were primed with TNF-α for 20 min and the other 50% for 1 min (B; dashed line). The production of superoxide anions was measured by isoluminol-amplified CL as described above. The figure shows the kinetics of representative experiments and the ratios between the peak responses in the populations are also given.