Figure 8

CR3-upregulation in TNF-α primed neutrophils. Neutrophils were incubated with TNF-α (25 ng/ml, 20 min, 37°C, solid line) and were paraformaldehyde-fixed, incubated with phycoerythrin-conjugated anti-CR3 antibodies (CD18/CD11b) and analysed by flow cytometry and compared with control cells (dashed line). Representative histograms of binding are shown, and the inset shows the exposure of CR3 on fMLF-primed neutrophils. The numbers indicate the mean fluorescence intensity as percent of control ± SD, n = 3.