Figure 9
From: Cytochalasin B triggers a novel pertussis toxin sensitive pathway in TNF-alpha primed neutrophils
CR3-upregulation in IL-8 and TNF-α primed neutrophils. Neutrophils were incubated with or without IL-8 (100 ng/ml) or TNF-α (25 ng/ml) for 20 min at 37°C. The cells were then paraformaldehyde-fixed, incubated with phycoeryterin-conjugated anti-CR3 antibodies (CD18/CD11b), analysed by flow cytometry and compared with control cells. The data is calculated from the mean fluorescence intensity of each cell population and expressed as percentage of the value obtained in control cells, n = 6.