Cytochalasin B triggers a novel pertussis toxin sensitive pathway in TNF-alpha primed neutrophils

Table 1 Deactivation/reactivation properties of classical chemoattractants and their receptors.

Effects on the neutrophil NADPH-oxidase*
Agonist (conc.)	Receptor(s)	Activation	Deactivation	Reactivation
fMLF (l0^-7M)	FPR	+	+	+
WKYMVM (10^-7M)	FPRL1	+	+	+
C5a (100 ng/ml)	C5aR	+	+	+
PAF (10^-7M)	PAFR	+	+	-
IL8 (100 ng/ml)	CXCR 1/2	+	+	-

*Indicated chemoattractants were used to stimulate human neutrophils directly (activation), or added to the cells at 15 (according to the protocol described in Methods) after which these cells were transferred to 37 and stimulated with the same agonist (deactivation) or reactivated with cytochalasin B (reactivation). The superoxide anion production was measured by isolummol-enhanced CL. The + and - indicate whether or not the process occurred. The experiments were repeated between 2–10 times.

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