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Figure 1 | BMC Cell Biology

Figure 1

From: The XMAP215-family protein DdCP224 is required for cortical interactions of microtubules

Figure 1

Overexpression of DdCP224-ΔC-GFP causes a collapse of interphase microtubule arrays. (A, B, B') Confocal microscopy of GFP-α-tubulin cells (A; control) and DdCP224-ΔC-GFP cells showing brightest point projections of GFP fluorescence (A, B') or immunofluorescence staining (B) using the YL1/2 anti-tubulin antibody (Chemicon, Hofheim, Germany). Cells were fixed with methanol. DNA was stained with TOPRO3 (Molecular Probes, Hilversum, Netherlands) (blue). (C) Immunoblot of a cytosolic extract of DdCP224-ΔC-GFP cells stained with anti-DdCP-HindIII. This rabbit polyclonal antibody was raised against the recombinant His-tagged N-terminus of DdCP224 using the 5'-HindIII fragment of the DdCP224 coding sequence. As calculated by the ImageJ program, DdCP224-ΔC-GFP is overexpressed approximately 5-fold. Bar 2 μm.

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