Skip to main content
Figure 5 | BMC Cell Biology

Figure 5

From: The XMAP215-family protein DdCP224 is required for cortical interactions of microtubules

Figure 5

Coprecipitation of DdCP224 with dynein and DdEB1. Experiments were performed using cytosolic extracts from wildtype cells (strain AX2) (A-D), GFP-Ddp62 cells (E) and DdCP224-ΔC-GFP cells (F). The respective co-immunoprecipitation (Co-IP) experiment is given in bold letters on top of each subfigure. The respective antibodies used for immunoprecipitation and staining of the immunoblots are indicated. Abbreviations and antibodies: DHC, anti-dynein heavy chain Y7 [19]; DIC, anti-dynein intermediate chain [20]; DdCP, anti-DdCP224 mAb 2/165 [35] for immunoblot staining and anti-DdCP-HindIII for immunoprecipitation; DdEB1, anti-DdEB1 [16]; p62, anti-Ddp62; control, anti-rabbit or anti-rat (in case of DIC) preimmune serum.

Back to article page