Figure 3

Alignment of the conserved core of human FIH-1 with the homologous region from Hydra -PSR Alignment of the conserved core of human FIH-1 forming the central β-barrel structure that harbours the active site with the homologous region from Hydra-PSR. Extended β-strands are depicted by blue arrows, for Hydra-PSR above the sequence, for human FIH underneath and numbered according to the published crystal structure [15]. Yellow boxes indicate the residues necessary to complex the active site Fe²⁺ ion, violet box indicates the lysine implicated in 2-oxo-glutarate binding. The hydrophobic residues that stabilise the barrel structure are highlighted in grey. Stars indicate residues within 4 Angstrom of the catalytic H-X-D that constitute the active site.