Figure 4
Localization of VHA-subunits in plant cells, using anti-VHA-A, anti-VHA-E and anti-VHA-a Nterm -antibody Confocal images represent single images of isolated maize root cells. Immuno-staining was performed on maize root cells. Secondary fluorescently labelled antibodies used were anti-rabbit-FITC (A-F) or anti-guineapig (Cy5). Images were colour coded with Adobe Photoshop. Scalebars are 10 μm. Labelling of tonoplast membranes with anti VHA-A (A to C) and anti VHA-E (D-F) in root cells of increasing age, i.e. non-vacuolized to vacuolized (left to right). Anti-VHA-aN-term labelling of root cells of increasing age (G-I). Note that the staining pattern of VHA-aN-term is distinct from the tonoplast labelling with VHA-A and VHA-E in all cases.