Figure 7
From: Cytoskeletal influences on nuclear shape in granulocytic HL-60 cells
Confocal immunofluorescent images of HL-60- bcl -2 cells. A. Gallery of undifferentiated and RA treated (7 days) cells. The columns (left to right) are: anti-centromere (CREST); anti-dimethyl H3K9; anti-nucleolus; anti-Golgi p58; anti-calreticulin. LMNB is always shown in green; the different antigens in red. Scale bar: 10 μm. B. Stereo image of undifferentiated HL-60-bcl-2 cells stained with anti-centromere (red), anti-LMNB (blue) and anti-LBR (green). Note the presence of centromeres within a micronucleus, which exhibits a deficiency of LBR. Scale bar: 10 μm.