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Figure 3 | BMC Cell Biology

Figure 3

From: Mutations in the IGF-II pathway that confer resistance to lytic reovirus infection

Figure 3

CTCF and Igf2 expression in RIE-1 and 6B72 cells. Levels of CTCF protein were assessed by Western blot analysis (A), normalized to a β-actin control. Levels of Igf2 transcripts were assessed by Northern blot analysis (B) normalized to GAPDH control. Protein content, as assayed by western blot analysis and standardized to β-actin was decreased in the 6B72 cell clone to 30% of control. Reverse transcriptase PCR analysis of Igf2 transcripts (C). The RT-PCR products (arrows) were separated on a 2% agarose gel, revealing an additional transcript in the 6B72 cells. The DNA sequence of the larger RT-PCR product (D) revealed an alternatively spliced transcript (Igf2sv) generated by splicing of exon 2 to a cryptic 3' splice site located 14 nucleotides upstream of exon 3.

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