Figure 8From: Mutations in the IGF-II pathway that confer resistance to lytic reovirus infection6B72 cells have delayed disassembly of reovirus type 1. Fluorescein-labelled reovirus particles were absorbed to RIE-1 (A) or 6B72 cells (B). Persistent fluorescence at 2 hours was found in 6B72 cells, but not in RIE-1 cells. Non-replicating reovirus type 1, at 3 × 104 particles per cell, was adsorbed to RIE-1 (C) or 6B72 (D) cells at 4°C, washed and incubated at 37°C for 2 and 4 hours. Cells were lysed and the state of virus particles determined by western blot. The outer capsid proteins μ1 and σ3 are present in the 6B72 cell preparations at 2 and 4 hours, but not in the RIE-1 cells.Back to article page