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Figure 9 | BMC Cell Biology

Figure 9

From: Mutations in the IGF-II pathway that confer resistance to lytic reovirus infection

Figure 9

Anchorage-independent growth phenotypes of RIE-1 cell clones. 105 cells were suspended in media containing 1% agarose and plated in 6 well culture dishes. RIE-1 cells acquire the ability to grow in soft agar after being transfected with a vector expressing pro-IGF2 but not the IGF2SV splice-variant (a). The vector inserted in the Ctcf gene (6B72) confers the ability to grow in soft agar, but the phenotype is suppressed by expression of IGF2SV (b). Clones selected for reovirus resistance with gene trap vectors inserted into the Prss11, Igf2r and Anxa2 genes failed to grow in soft agar (c). RIE-1 cells expressing native IGF-II protein without the E-peptide grew in soft agar but the colonies were smaller (d) than produced by pro-IGF-2 (a), while the corresponding IGF-IIA62T protein (E-peptide) did not transform RIE-1 cells to anchorage independence (d). Colonies were photographed (20×) after 7 days except (d) where the cells were photographed after 10 days.

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