Figure 7

Effect of PKCζ mutants on p38 phosphorylation. A, Cells were transiently transfected with wt PKCζ, kinase-deficient T410A PKCζ and constitutively active T410E PKCζ for 48 h and treated with or without PHE for 5 min. Phospho-p38 and p38 protein levels in cell lysates were determined by western blot analysis as described in Methods. The bar graph (B) represents quantification of the ratio phospho-p38 / 38 protein bands by densitometric analysis of blots from four different experiments. * denotes value significantly different from vehicle (nontransfected and lipofectamine-treated cells), p < .01.