Figure 2

RIE-1 mutant cells resist lytic infection by reovirus The columns contain an unselected RIE-1 cell library, RIE-1 40C, and representative reovirus resistant mutant cell clones. Serial two-fold dilutions of reovirus were made with the highest titer in the upper row, MOI = 1 × 10⁴. Resistance to reovirus type 1 infection was observed in the mutant cells 3 to 7 days post-infection. The bottom row of cells, denoted by "C", were not infected to serve as controls for cell viability and proliferation. Cells were stained with gentian violet four days post-infection. A clear well indicates cell death following virus infection.