Figure 3From: Isoform-specific expression of the Coxsackie and adenovirus receptor (CAR) in neuromuscular junction and cardiac intercalated discsImmunolocalization of CAR to the neuromuscular junction in mouse skeletal muscle. Frozen sections of mouse muscle were incubated with Alexa Fluor-conjugated α-bungarotoxin and antibodies to CAR as described in Materials and Methods. Panels A and D show α-bungarotoxin staining of acetylcholine receptors at neuromuscular junctions (green). Panel B shows immunofluorescent staining of the section in panel A with a polyclonal antibody (ab 2240) to the extracellular domain of CAR (red). Panel E shows immunofluorescent staining of the section in panel D with a chicken polyclonal antibody (ChCT) directed against a C-terminal portion of CAR conserved in both CAR isoforms (red). Panel C is a merge of panels A and B. Panel F is a merge of panels D and E. These merges [C, F] show that CAR colocalizes with α-bungarotoxin at murine neuromuscular junctions (in yellow). Sections incubated with the secondary antibodies alone did not give any signal – anti-rabbit IgG [G], anti-chicken IgY [H]. Bar = 35 μmBack to article page