Figure 2

Sub-cellular localisation of epitope-tagged lamin As. A) Detection of FLAG-tagged wt and mutant lamin As transfected into human HT1080 fibrosarcoma cells. The FLAG tag was detected by immunofluorescence with M2 anti-FLAG (red in merge), in DAPI stained nuclei (blue in merge). Bar = 10 μm. B) Detection of GFP-tagged wt and mutant lamin As transfected into human HT1080 fibrosarcoma cells. GFP signal in images collected at 2 μm intervals from the top to the bottom of the nucleus is shown in black and white. The merged colour images (far right) show mid-plane images of the GFP signal (green) in DAPI stained nuclei (blue). Bar = 10 μm.